Oxidative modification of glyceraldehyde-3-phosphate dehydrogenase influences its interaction with endogenous neuroprotector isatin
نویسندگان
چکیده
منابع مشابه
Isatin interaction with glyceraldehyde-3-phosphate dehydrogenase, a putative target of neuroprotective drugs: partial agonism with deprenyl.
There is evidence that the binding of deprenyl, a monoamine oxidase (MAO) B inhibitor, and other propargylamines to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is primarily responsible for their neuroprotective and antiapoptotic effects. Thus, GAPDH may be a target for other neuroprotective drugs. Using two independent approaches, radioligand analysis and an optical biosensor technique, we...
متن کاملDifferential protein S-thiolation of glyceraldehyde-3-phosphate dehydrogenase isoenzymes influences sensitivity to oxidative stress.
The irreversible oxidation of cysteine residues can be prevented by protein S-thiolation, in which protein -SH groups form mixed disulfides with low-molecular-weight thiols such as glutathione. We report here the identification of glyceraldehyde-3-phosphate dehydrogenase as the major target of protein S-thiolation following treatment with hydrogen peroxide in the yeast Saccharomyces cerevisiae....
متن کاملLiposome-interaction induced conformation changes of glyceraldehyde-3-phosphate dehydrogenase.
Tryptophanyl emission spectra of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were measured after the addition of liposomes prepared of natural phospholipids: phosphatidylinositols (PI), phosphatidylserines (PS) and phosphatidylcholines (PC). The measurings were made for various molar lipid/protein ratios (100-1000). A decrease in the enzyme fluorescence intensity and a "red" ...
متن کاملPhosphoglycerate kinase--glyceraldehyde-3-phosphate dehydrogenase interaction: reaction rate studies.
Rate studies using phosphoglycerate kinase (PGK)--glyceraldehyde-3-phosphate dehydrogenase (GPDH) enzyme pair have been carried out to distinguish between the two mechanisms of intermediate metabolite transfer, namely diffusion through the solvent versus "substrate channelling" within an enzyme-enzyme complex. A procedure has been described for the assay of the rates of PGK-catalysed and the PG...
متن کاملOxidative modifications of glyceraldehyde-3-phosphate dehydrogenase play a key role in its multiple cellular functions.
Knowledge of the cellular targets of ROS (reactive oxygen species) and their regulation is an essential prerequisite for understanding ROS-mediated signalling. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is known as a major target protein in oxidative stresses and becomes thiolated in its active site. However, the molecular and functional changes of oxidized GAPDH, the inactive form, have ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomeditsinskaya Khimiya
سال: 2016
ISSN: 2310-6905,2310-6972
DOI: 10.18097/pbmc20166202160